Puromycin Incorporation as a Measure of Global Protein Synthesis
Andrew R. Kelleher, PhD Candidate, Scot Kimball Lab, Penn State College of Medicine — Published: September 30, 2013 (A) C2C12 myoblasts were starved of serum and leucine for 2 hr and then IGF-1 and leucine were added to the medium of some of the cells for 45 min. Puromycin (1uM) was added to the