Hitting upon an optimal G Protein alpha purification scheme

Hitting upon an optimal G Protein alpha purification scheme

Gregory G. Tall, PhD, University of Rochester — Published: April 11, 2013 Ric-8A promotes Gαq abundance. Lysates from Sf9 cells expressing Gαq or Gαq and Ric-8A were chromatographed and the Gαq-containing fractions were resolved by SDS-PAGE and Coomassie stained. Our ability to produce unprecedented quantity of heterotrimeric G protein α subunits was not by design, 

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