Hitting upon an optimal G Protein alpha purification scheme
Gregory G. Tall, PhD, University of Rochester — Published: April 11, 2013 Ric-8A promotes Gαq abundance. Lysates from Sf9 cells expressing Gαq or Gαq and Ric-8A were chromatographed and the Gαq-containing fractions were resolved by SDS-PAGE and Coomassie stained. Our ability to produce unprecedented quantity of heterotrimeric G protein α subunits was not by design,